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Forensic DNA typing : biology & technology behind STR markers / John M. Butler.

By: Material type: TextTextPublication details: San Diego : Elsevier Academic Press, c2001.Description: xii, 322 p. : ill. (some col.) ; 25 cmISBN:
  • 012147951X
  • 9780121479510:
Subject(s): DDC classification:
  • 614.1
Contents:
ch. 1. Overview and history of DNA typing -- ch. 2. DNA biology review -- ch. 3. Sample collection and preparation (DNA extraction and quantification -- ch. 4. The polymerase chain reaction (DNA amplification) -- ch. 5. Commonly used short tandem repeat markers -- ch. 6. Biology of STRs : stutter products, non-template addition, microvariants, null alleles, and mutation rates -- ch. 7. Forensic issues : degraded DNA, PCR inhibition, contamination, and mixed samples -- ch. 8. Additional DNA markers : amelogenin, y-chromosome STRs, mtDNA, SNPs, Alu repeats -- ch. 9. DNA separation methods : slab-gel and capillary electrophoresis -- ch. 10. DNA detection methods : fluorescent dyes and silver staining -- ch. 11. The ABI prism 310 genetic analyzer -- ch. 12. The Hitachi FMBIO II fluorescence imaging system -- ch. 13. STR genotyping issues -- ch. 14. Laboratory validation -- ch. 15. New technologies and automation -- ch. 16. Combined DNA index system (codis) and the use of DNA databases -- ch. 17. DNA testing in high-profile cases.
Holdings
Item type Current library Call number Copy number Status Date due Barcode
General Lending Carlow Campus Library General Lending 614.1 (Browse shelf(Opens below)) 1 Available 45790

Includes bibliographical references and index.

ch. 1. Overview and history of DNA typing -- ch. 2. DNA biology review -- ch. 3. Sample collection and preparation (DNA extraction and quantification -- ch. 4. The polymerase chain reaction (DNA amplification) -- ch. 5. Commonly used short tandem repeat markers -- ch. 6. Biology of STRs : stutter products, non-template addition, microvariants, null alleles, and mutation rates -- ch. 7. Forensic issues : degraded DNA, PCR inhibition, contamination, and mixed samples -- ch. 8. Additional DNA markers : amelogenin, y-chromosome STRs, mtDNA, SNPs, Alu repeats -- ch. 9. DNA separation methods : slab-gel and capillary electrophoresis -- ch. 10. DNA detection methods : fluorescent dyes and silver staining -- ch. 11. The ABI prism 310 genetic analyzer -- ch. 12. The Hitachi FMBIO II fluorescence imaging system -- ch. 13. STR genotyping issues -- ch. 14. Laboratory validation -- ch. 15. New technologies and automation -- ch. 16. Combined DNA index system (codis) and the use of DNA databases -- ch. 17. DNA testing in high-profile cases.

69.08

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