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005 | 20201215152917.0 | ||
008 | 900823s1990 cau b 000 0 eng | ||
010 | _z54009110 | ||
020 | _a0121820831 (hardcover : alk. paper) : 0122135857 (comb bound : alk. paper) | ||
020 | _a0122135857 (comb bound : alk. paper) | ||
020 |
_a9780121820831: _cNo price |
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_aQP601.M49 _bv.182 |
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072 | 7 |
_aPSB _2thema |
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072 | 7 |
_aPDN _2thema |
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_aPN _2thema |
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082 | _a572.6 | ||
245 | 0 | 0 |
_aGuide to protein purification / _cedited by Murray P. Deutscher. |
260 |
_aSan Diego : _bAcademic Press, _cc1990. |
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300 |
_axxix, 894 p. : _bill. ; _c25cm. ; _epbk. comb binding |
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490 | 0 |
_aMethods in enzymology ; _vv. 182 |
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500 | _aIncludes indexes. | ||
504 | _aIncludes bibliographical references. | ||
505 | 0 | _tWhy purify enzymes? / Arthur Kornberg. | |
505 | 0 |
_a[Section I Developing Purification Procedures] _tStrategies and considerations for protein purification / Stuart Linn. |
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505 | 0 |
_a[Section II General Methods for Handling Proteins and Enzymes] _tSetting up a laboratory / Murray P. Deutscher -- _tBuffers: principles and practice / Vincent S. Stoll & John S. Blanchard -- _tMeasurement of enzyme activity / Edward F. Rossomando -- _tQuantitation of protein / Christa M. Stoscheck -- _tConcentration of proteins and removal of solutes / Thomas Pohl -- _tMaintaining protein stability / Murray P. Deutscher. |
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505 | 0 |
_a[Section III Optimization of Starting Materials] _tOverproduction of proteins in Escherichia coli: vectors, hosts and strategies / Asis Das -- _tOverexpression of proteins in eukaryotes / Margaret K. Bradley -- _tSecretion of heterologous proteins in Escherichia coli / I. Barry Holland et al. |
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505 | 0 |
_a[Section IV Preparation of Extracts and Subcellular Fractionation] _tPreparation of extracts from prokaryotes / Millard Cull and Charles S. McHenry -- _tPreparation of extracts from yeast / S. Michal Jazwinski -- _tPreparation of extracts from plants / Peter Gegenheimer -- _tPreparation of extracts from higher eukaryotes / John David Dignam -- _tIsolation of subcellular organelles / Brian Storrie & Edward A. Madden -- _tPreparation of membrane fractions / Juris Ozols. |
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505 | 0 |
_a[Section V Solubilization Procedures] _tDetergents: an overview / Judith M. Neugebauer -- _tSolubilization of native membrane proteins / Leonard M. Hjelmeland -- _tSolubilization of protein aggretates / Fiona A. O. Marston & Donal L. Hartley -- _tRemoval of detergents from membrane proteins / Leonard M. Hjelmeland. |
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505 | 0 |
_a[Section VI Purification Procedures: Bulk Methods] _tPrecipitation techniques / Sasha Englard & Sam Seifter -- _tPrecipitation of proteins with polyethylene glycol / Kenneth C. Ingham. |
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505 | 0 |
_a[Section VII Purification Procedures: Chromatographic Methods] _tIon-exchange chromatography / Edward F. Rossomando -- _tGel filtration / Earle Stellwagen -- _tProtein chromatography on hydroxyapatite columns / Marina J. Gorbunoff -- _tHydrophobic chromatography / Robert M. Kennedy -- _tChromatography on immobilized reactive dyes / Earle Stellwagen -- _tAffinity chromatography: general methods / Steven Ostrove -- _tAffinity chromatography: specialized techniques / Steven Ostrove & Shelly Weiss -- _tChromatofocusing / Lallan Giri -- _tHigh-performance liquid chromatography: effective protein purification by various chromatograpic modes / Roman M. Chicz & Fred E. Regnier. |
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505 | 0 |
_a[Section VIII Purification Procedures: Electrophoretic Methods] _tOne-dimensional gel electrophoresis / David E. Garfin -- _tProtein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis / Bonnie S. Dunbar, Hitomi Kimura & Therese M. Timmons -- _tIsoelectric focusing / David E. Garfin -- _tGel staining techniques / Carl R. Merril -- _tEvolution of protein from gels / Michael G. Harrington. |
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505 | 0 |
_a[Section IX Specialized Purification Procedures] _tPurification of membrane proteins / Thomas C. Thomas & Mark G. McNamee -- _tPurification of DNA-binding proteins by site-specific DNA affinity chromatography / Shlomo Eisenberg et al -- _tPurification of glycoproteins / Craig Gerard -- _tPurification of multienzyme complexes / Paul A. Srere & Christopher K. Mathews. |
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505 | 0 |
_a[Section X Characterization of Purified Proteins] _tDetermination of purity / David G. Rhodes & Thomas M. Laue -- _tDetermination of size, molecular weight and presence of subunits / Thomas M. Laue & David G. Rhodes -- _tAmino acid analysis / Juris Ozols -- _tLimited N-terminal sequence analysis / Paul Matsudaira -- _tPeptide mapping / Ralph C. Judd -- _tAnalysis for protein modifications and nonprotein cofactors / Sam Seifter & Sasha Englard -- _tProtein crystallization / David Ollis & Stephen White. |
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505 | 0 |
_a[Section XI Immunological Procedures] _tPreparation of polyclonal antibodies / Bonnie S. Dunbar & Eric D. Schwoebel -- _tPreparation of monoclonal antibodies / Bonnie S. Dunbar & Sheri M. Skinner -- _tProtein blotting and immunodetection / Therese M. Timmons & Bonnie S. Dunbar -- _tImmunoprecipitation of proteins / Gary L. Firestone & Sandra D. Winguth -- _tImmunoassays / Charles W. Parker. |
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505 | 0 |
_a[Section XII Additional Techniques] _tRadiolabeling of proteins / Charles W. Parker -- _tUsing purified protein to clone its gene / John M. Wozney -- _tComputer analysis of protein structure / Patrick Argos. |
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505 | 0 |
_a[Section XIII Concluding Remarks] _tRethinking your purification procedure / Murray P. Deutscher. |
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505 | 0 |
_a[Appendix Personal Perspectives on Enzyme Purification] _tThe private life of DNA polymerase I / Arthue Kornberg -- _tPurification and crystallization of transaldolase from candida utilis / B. L. Horecker & O. Tsolas -- _tDiscovery of glutamine synthetase cascade / Earl R. Stadtman -- _tDiscovery, resolution, purification and function of elongation factors / Kivie Moldave. |
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590 | _a81.74 | ||
650 | 0 |
_aProteins _xPurification _947746 |
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650 | 0 |
_aProteins _xIsolation & purification _968463 |
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650 | 0 |
_aEnzymes. _968464 |
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650 | 7 |
_aScience. _2ukslc _968465 |
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650 | 7 |
_aBiochemistry _2thema _968466 |
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650 | 7 |
_aScientific equipment, experiments & techniques _2thema _934492 |
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650 | 7 |
_aChemistry _2thema _968467 |
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700 | 1 |
_aDeutscher, Murray P. _929809 |
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