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008 900823s1990 cau b 000 0 eng
010 _z54009110
020 _a0121820831 (hardcover : alk. paper) : 0122135857 (comb bound : alk. paper)
020 _a0122135857 (comb bound : alk. paper)
020 _a9780121820831:
_cNo price
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_cNLM
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_dIPL
_dZHM
_dVET
_dMUQ
070 0 _aQP601.M49
_bv.182
072 0 _aX300
072 7 _aPSB
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072 7 _aPDN
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072 7 _aPN
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082 _a572.6
245 0 0 _aGuide to protein purification /
_cedited by Murray P. Deutscher.
260 _aSan Diego :
_bAcademic Press,
_cc1990.
300 _axxix, 894 p. :
_bill. ;
_c25cm. ;
_epbk. comb binding
490 0 _aMethods in enzymology ;
_vv. 182
500 _aIncludes indexes.
504 _aIncludes bibliographical references.
505 0 _tWhy purify enzymes? / Arthur Kornberg.
505 0 _a[Section I Developing Purification Procedures]
_tStrategies and considerations for protein purification / Stuart Linn.
505 0 _a[Section II General Methods for Handling Proteins and Enzymes]
_tSetting up a laboratory / Murray P. Deutscher --
_tBuffers: principles and practice / Vincent S. Stoll & John S. Blanchard --
_tMeasurement of enzyme activity / Edward F. Rossomando --
_tQuantitation of protein / Christa M. Stoscheck --
_tConcentration of proteins and removal of solutes / Thomas Pohl --
_tMaintaining protein stability / Murray P. Deutscher.
505 0 _a[Section III Optimization of Starting Materials]
_tOverproduction of proteins in Escherichia coli: vectors, hosts and strategies / Asis Das --
_tOverexpression of proteins in eukaryotes / Margaret K. Bradley --
_tSecretion of heterologous proteins in Escherichia coli / I. Barry Holland et al.
505 0 _a[Section IV Preparation of Extracts and Subcellular Fractionation]
_tPreparation of extracts from prokaryotes / Millard Cull and Charles S. McHenry --
_tPreparation of extracts from yeast / S. Michal Jazwinski --
_tPreparation of extracts from plants / Peter Gegenheimer --
_tPreparation of extracts from higher eukaryotes / John David Dignam --
_tIsolation of subcellular organelles / Brian Storrie & Edward A. Madden --
_tPreparation of membrane fractions / Juris Ozols.
505 0 _a[Section V Solubilization Procedures]
_tDetergents: an overview / Judith M. Neugebauer --
_tSolubilization of native membrane proteins / Leonard M. Hjelmeland --
_tSolubilization of protein aggretates / Fiona A. O. Marston & Donal L. Hartley --
_tRemoval of detergents from membrane proteins / Leonard M. Hjelmeland.
505 0 _a[Section VI Purification Procedures: Bulk Methods]
_tPrecipitation techniques / Sasha Englard & Sam Seifter --
_tPrecipitation of proteins with polyethylene glycol / Kenneth C. Ingham.
505 0 _a[Section VII Purification Procedures: Chromatographic Methods]
_tIon-exchange chromatography / Edward F. Rossomando --
_tGel filtration / Earle Stellwagen --
_tProtein chromatography on hydroxyapatite columns / Marina J. Gorbunoff --
_tHydrophobic chromatography / Robert M. Kennedy --
_tChromatography on immobilized reactive dyes / Earle Stellwagen --
_tAffinity chromatography: general methods / Steven Ostrove --
_tAffinity chromatography: specialized techniques / Steven Ostrove & Shelly Weiss --
_tChromatofocusing / Lallan Giri --
_tHigh-performance liquid chromatography: effective protein purification by various chromatograpic modes / Roman M. Chicz & Fred E. Regnier.
505 0 _a[Section VIII Purification Procedures: Electrophoretic Methods]
_tOne-dimensional gel electrophoresis / David E. Garfin --
_tProtein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis / Bonnie S. Dunbar, Hitomi Kimura & Therese M. Timmons --
_tIsoelectric focusing / David E. Garfin --
_tGel staining techniques / Carl R. Merril --
_tEvolution of protein from gels / Michael G. Harrington.
505 0 _a[Section IX Specialized Purification Procedures]
_tPurification of membrane proteins / Thomas C. Thomas & Mark G. McNamee --
_tPurification of DNA-binding proteins by site-specific DNA affinity chromatography / Shlomo Eisenberg et al --
_tPurification of glycoproteins / Craig Gerard --
_tPurification of multienzyme complexes / Paul A. Srere & Christopher K. Mathews.
505 0 _a[Section X Characterization of Purified Proteins]
_tDetermination of purity / David G. Rhodes & Thomas M. Laue --
_tDetermination of size, molecular weight and presence of subunits / Thomas M. Laue & David G. Rhodes --
_tAmino acid analysis / Juris Ozols --
_tLimited N-terminal sequence analysis / Paul Matsudaira --
_tPeptide mapping / Ralph C. Judd --
_tAnalysis for protein modifications and nonprotein cofactors / Sam Seifter & Sasha Englard --
_tProtein crystallization / David Ollis & Stephen White.
505 0 _a[Section XI Immunological Procedures]
_tPreparation of polyclonal antibodies / Bonnie S. Dunbar & Eric D. Schwoebel --
_tPreparation of monoclonal antibodies / Bonnie S. Dunbar & Sheri M. Skinner --
_tProtein blotting and immunodetection / Therese M. Timmons & Bonnie S. Dunbar --
_tImmunoprecipitation of proteins / Gary L. Firestone & Sandra D. Winguth --
_tImmunoassays / Charles W. Parker.
505 0 _a[Section XII Additional Techniques]
_tRadiolabeling of proteins / Charles W. Parker --
_tUsing purified protein to clone its gene / John M. Wozney --
_tComputer analysis of protein structure / Patrick Argos.
505 0 _a[Section XIII Concluding Remarks]
_tRethinking your purification procedure / Murray P. Deutscher.
505 0 _a[Appendix Personal Perspectives on Enzyme Purification]
_tThe private life of DNA polymerase I / Arthue Kornberg --
_tPurification and crystallization of transaldolase from candida utilis / B. L. Horecker & O. Tsolas --
_tDiscovery of glutamine synthetase cascade / Earl R. Stadtman --
_tDiscovery, resolution, purification and function of elongation factors / Kivie Moldave.
590 _a81.74
650 0 _aProteins
_xPurification
_947746
650 0 _aProteins
_xIsolation & purification
_968463
650 0 _aEnzymes.
_968464
650 7 _aScience.
_2ukslc
_968465
650 7 _aBiochemistry
_2thema
_968466
650 7 _aScientific equipment, experiments & techniques
_2thema
_934492
650 7 _aChemistry
_2thema
_968467
700 1 _aDeutscher, Murray P.
_929809
902 _a140901
903 _aMARS
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