| 000 | 04274nam a22006495i 4500 | ||
|---|---|---|---|
| 001 | 978-3-319-16259-1 | ||
| 003 | DE-He213 | ||
| 005 | 20201217111634.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 150508s2015 gw | s |||| 0|eng d | ||
| 020 |
_a9783319162591 _9978-3-319-16259-1 |
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| 024 | 7 |
_a10.1007/978-3-319-16259-1 _2doi |
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| 072 | 7 |
_aPSTL _2bicssc |
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| 072 | 7 |
_aSCI011000 _2bisacsh |
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| 072 | 7 |
_aPST _2thema |
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| 072 | 7 |
_aTCB _2thema |
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| 082 | 0 | 4 |
_a631.52 _223 |
| 082 | 0 | 4 |
_a660.6 _223 |
| 100 | 1 |
_aTill, Bradley J. _9103194 |
|
| 245 | 1 | 0 |
_aLow-Cost Methods for Molecular Characterization of Mutant Plants _h[electronic resource] : _bTissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / _cby Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger. |
| 250 | _a1st ed. 2015. | ||
| 264 | 1 |
_aCham : _bSpringer International Publishing : _bImprint: Springer, _c2015. |
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| 300 |
_aX, 35 p. 9 illus., 3 illus. in color. _bonline resource. |
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| 336 | _btxt | ||
| 337 | _bc | ||
| 338 | _bcr | ||
| 347 |
_atext file _bPDF _2rda |
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| 500 | _aIT Carlow ebook | ||
| 505 | 0 | _aIntroduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions. . | |
| 506 | 0 | _aOpen Access | |
| 520 | _aThis book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits. | ||
| 650 | 0 |
_aPlant breeding. _96462 |
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| 650 | 0 |
_aBiology-Technique. _997923 |
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| 650 | 0 |
_aNucleic acids. _95990 |
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| 650 | 1 | 4 |
_aPlant Breeding/Biotechnology. _0https://scigraph.springernature.com/ontologies/product-market-codes/L24060 _997665 |
| 650 | 2 | 4 |
_aBiological Techniques. _0https://scigraph.springernature.com/ontologies/product-market-codes/L28000 _997929 |
| 650 | 2 | 4 |
_aNucleic Acid Chemistry. _0https://scigraph.springernature.com/ontologies/product-market-codes/L14011 _9103195 |
| 700 | 1 |
_aJankowicz-Cieslak, Joanna. _9103196 |
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| 700 | 1 |
_aHuynh, Owen A. _9103197 |
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| 700 | 1 |
_aBeshir, Mayada M. _9103198 |
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| 700 | 1 |
_aLaport, Robert G. _9103199 |
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| 700 | 1 |
_aHofinger, Bernhard J. _9103200 |
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| 710 | 2 |
_aSpringerLink (Online service) _930940 |
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| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9783319162607 |
| 776 | 0 | 8 |
_iPrinted edition: _z9783319162584 |
| 776 | 0 | 8 |
_iPrinted edition: _z9783319386676 |
| 856 | 0 |
_ySend a message to library staff if access to this online resource is unavailable _uhttps://tinyurl.com/y2hljxwd |
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| 856 | 4 | 0 |
_yLink to Springer open access ebook _uhttps://doi.org/10.1007/978-3-319-16259-1 |
| 912 | _aZDB-2-SBL | ||
| 912 | _aZDB-2-SXB | ||
| 912 | _aZDB-2-SOB | ||
| 999 |
_c50139 _d50139 |
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