Guide to protein purification /
Guide to protein purification /
edited by Murray P. Deutscher.
- San Diego : Academic Press, c1990.
- xxix, 894 p. : ill. ; 25cm. ; pbk. comb binding
- Methods in enzymology ; v. 182 .
Includes indexes.
Includes bibliographical references.
Why purify enzymes? / Arthur Kornberg. [Section I Developing Purification Procedures] Strategies and considerations for protein purification / Stuart Linn. [Section II General Methods for Handling Proteins and Enzymes] Setting up a laboratory / Murray P. Deutscher -- Buffers: principles and practice / Vincent S. Stoll & John S. Blanchard -- Measurement of enzyme activity / Edward F. Rossomando -- Quantitation of protein / Christa M. Stoscheck -- Concentration of proteins and removal of solutes / Thomas Pohl -- Maintaining protein stability / Murray P. Deutscher. [Section III Optimization of Starting Materials] Overproduction of proteins in Escherichia coli: vectors, hosts and strategies / Asis Das -- Overexpression of proteins in eukaryotes / Margaret K. Bradley -- Secretion of heterologous proteins in Escherichia coli / I. Barry Holland et al. [Section IV Preparation of Extracts and Subcellular Fractionation] Preparation of extracts from prokaryotes / Millard Cull and Charles S. McHenry -- Preparation of extracts from yeast / S. Michal Jazwinski -- Preparation of extracts from plants / Peter Gegenheimer -- Preparation of extracts from higher eukaryotes / John David Dignam -- Isolation of subcellular organelles / Brian Storrie & Edward A. Madden -- Preparation of membrane fractions / Juris Ozols. [Section V Solubilization Procedures] Detergents: an overview / Judith M. Neugebauer -- Solubilization of native membrane proteins / Leonard M. Hjelmeland -- Solubilization of protein aggretates / Fiona A. O. Marston & Donal L. Hartley -- Removal of detergents from membrane proteins / Leonard M. Hjelmeland. [Section VI Purification Procedures: Bulk Methods] Precipitation techniques / Sasha Englard & Sam Seifter -- Precipitation of proteins with polyethylene glycol / Kenneth C. Ingham. [Section VII Purification Procedures: Chromatographic Methods] Ion-exchange chromatography / Edward F. Rossomando -- Gel filtration / Earle Stellwagen -- Protein chromatography on hydroxyapatite columns / Marina J. Gorbunoff -- Hydrophobic chromatography / Robert M. Kennedy -- Chromatography on immobilized reactive dyes / Earle Stellwagen -- Affinity chromatography: general methods / Steven Ostrove -- Affinity chromatography: specialized techniques / Steven Ostrove & Shelly Weiss -- Chromatofocusing / Lallan Giri -- High-performance liquid chromatography: effective protein purification by various chromatograpic modes / Roman M. Chicz & Fred E. Regnier. [Section VIII Purification Procedures: Electrophoretic Methods] One-dimensional gel electrophoresis / David E. Garfin -- Protein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis / Bonnie S. Dunbar, Hitomi Kimura & Therese M. Timmons -- Isoelectric focusing / David E. Garfin -- Gel staining techniques / Carl R. Merril -- Evolution of protein from gels / Michael G. Harrington. [Section IX Specialized Purification Procedures] Purification of membrane proteins / Thomas C. Thomas & Mark G. McNamee -- Purification of DNA-binding proteins by site-specific DNA affinity chromatography / Shlomo Eisenberg et al -- Purification of glycoproteins / Craig Gerard -- Purification of multienzyme complexes / Paul A. Srere & Christopher K. Mathews. [Section X Characterization of Purified Proteins] Determination of purity / David G. Rhodes & Thomas M. Laue -- Determination of size, molecular weight and presence of subunits / Thomas M. Laue & David G. Rhodes -- Amino acid analysis / Juris Ozols -- Limited N-terminal sequence analysis / Paul Matsudaira -- Peptide mapping / Ralph C. Judd -- Analysis for protein modifications and nonprotein cofactors / Sam Seifter & Sasha Englard -- Protein crystallization / David Ollis & Stephen White. [Section XI Immunological Procedures] Preparation of polyclonal antibodies / Bonnie S. Dunbar & Eric D. Schwoebel -- Preparation of monoclonal antibodies / Bonnie S. Dunbar & Sheri M. Skinner -- Protein blotting and immunodetection / Therese M. Timmons & Bonnie S. Dunbar -- Immunoprecipitation of proteins / Gary L. Firestone & Sandra D. Winguth -- Immunoassays / Charles W. Parker. [Section XII Additional Techniques] Radiolabeling of proteins / Charles W. Parker -- Using purified protein to clone its gene / John M. Wozney -- Computer analysis of protein structure / Patrick Argos. [Section XIII Concluding Remarks] Rethinking your purification procedure / Murray P. Deutscher. [Appendix Personal Perspectives on Enzyme Purification] The private life of DNA polymerase I / Arthue Kornberg -- Purification and crystallization of transaldolase from candida utilis / B. L. Horecker & O. Tsolas -- Discovery of glutamine synthetase cascade / Earl R. Stadtman -- Discovery, resolution, purification and function of elongation factors / Kivie Moldave.
0121820831 (hardcover : alk. paper) : 0122135857 (comb bound : alk. paper) 0122135857 (comb bound : alk. paper) 9780121820831: No price
Proteins--Purification
Proteins--Isolation & purification
Enzymes.
Science.
Biochemistry
Scientific equipment, experiments & techniques
Chemistry
572.6
QP601.M49 / v.182
Includes indexes.
Includes bibliographical references.
Why purify enzymes? / Arthur Kornberg. [Section I Developing Purification Procedures] Strategies and considerations for protein purification / Stuart Linn. [Section II General Methods for Handling Proteins and Enzymes] Setting up a laboratory / Murray P. Deutscher -- Buffers: principles and practice / Vincent S. Stoll & John S. Blanchard -- Measurement of enzyme activity / Edward F. Rossomando -- Quantitation of protein / Christa M. Stoscheck -- Concentration of proteins and removal of solutes / Thomas Pohl -- Maintaining protein stability / Murray P. Deutscher. [Section III Optimization of Starting Materials] Overproduction of proteins in Escherichia coli: vectors, hosts and strategies / Asis Das -- Overexpression of proteins in eukaryotes / Margaret K. Bradley -- Secretion of heterologous proteins in Escherichia coli / I. Barry Holland et al. [Section IV Preparation of Extracts and Subcellular Fractionation] Preparation of extracts from prokaryotes / Millard Cull and Charles S. McHenry -- Preparation of extracts from yeast / S. Michal Jazwinski -- Preparation of extracts from plants / Peter Gegenheimer -- Preparation of extracts from higher eukaryotes / John David Dignam -- Isolation of subcellular organelles / Brian Storrie & Edward A. Madden -- Preparation of membrane fractions / Juris Ozols. [Section V Solubilization Procedures] Detergents: an overview / Judith M. Neugebauer -- Solubilization of native membrane proteins / Leonard M. Hjelmeland -- Solubilization of protein aggretates / Fiona A. O. Marston & Donal L. Hartley -- Removal of detergents from membrane proteins / Leonard M. Hjelmeland. [Section VI Purification Procedures: Bulk Methods] Precipitation techniques / Sasha Englard & Sam Seifter -- Precipitation of proteins with polyethylene glycol / Kenneth C. Ingham. [Section VII Purification Procedures: Chromatographic Methods] Ion-exchange chromatography / Edward F. Rossomando -- Gel filtration / Earle Stellwagen -- Protein chromatography on hydroxyapatite columns / Marina J. Gorbunoff -- Hydrophobic chromatography / Robert M. Kennedy -- Chromatography on immobilized reactive dyes / Earle Stellwagen -- Affinity chromatography: general methods / Steven Ostrove -- Affinity chromatography: specialized techniques / Steven Ostrove & Shelly Weiss -- Chromatofocusing / Lallan Giri -- High-performance liquid chromatography: effective protein purification by various chromatograpic modes / Roman M. Chicz & Fred E. Regnier. [Section VIII Purification Procedures: Electrophoretic Methods] One-dimensional gel electrophoresis / David E. Garfin -- Protein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis / Bonnie S. Dunbar, Hitomi Kimura & Therese M. Timmons -- Isoelectric focusing / David E. Garfin -- Gel staining techniques / Carl R. Merril -- Evolution of protein from gels / Michael G. Harrington. [Section IX Specialized Purification Procedures] Purification of membrane proteins / Thomas C. Thomas & Mark G. McNamee -- Purification of DNA-binding proteins by site-specific DNA affinity chromatography / Shlomo Eisenberg et al -- Purification of glycoproteins / Craig Gerard -- Purification of multienzyme complexes / Paul A. Srere & Christopher K. Mathews. [Section X Characterization of Purified Proteins] Determination of purity / David G. Rhodes & Thomas M. Laue -- Determination of size, molecular weight and presence of subunits / Thomas M. Laue & David G. Rhodes -- Amino acid analysis / Juris Ozols -- Limited N-terminal sequence analysis / Paul Matsudaira -- Peptide mapping / Ralph C. Judd -- Analysis for protein modifications and nonprotein cofactors / Sam Seifter & Sasha Englard -- Protein crystallization / David Ollis & Stephen White. [Section XI Immunological Procedures] Preparation of polyclonal antibodies / Bonnie S. Dunbar & Eric D. Schwoebel -- Preparation of monoclonal antibodies / Bonnie S. Dunbar & Sheri M. Skinner -- Protein blotting and immunodetection / Therese M. Timmons & Bonnie S. Dunbar -- Immunoprecipitation of proteins / Gary L. Firestone & Sandra D. Winguth -- Immunoassays / Charles W. Parker. [Section XII Additional Techniques] Radiolabeling of proteins / Charles W. Parker -- Using purified protein to clone its gene / John M. Wozney -- Computer analysis of protein structure / Patrick Argos. [Section XIII Concluding Remarks] Rethinking your purification procedure / Murray P. Deutscher. [Appendix Personal Perspectives on Enzyme Purification] The private life of DNA polymerase I / Arthue Kornberg -- Purification and crystallization of transaldolase from candida utilis / B. L. Horecker & O. Tsolas -- Discovery of glutamine synthetase cascade / Earl R. Stadtman -- Discovery, resolution, purification and function of elongation factors / Kivie Moldave.
0121820831 (hardcover : alk. paper) : 0122135857 (comb bound : alk. paper) 0122135857 (comb bound : alk. paper) 9780121820831: No price
Proteins--Purification
Proteins--Isolation & purification
Enzymes.
Science.
Biochemistry
Scientific equipment, experiments & techniques
Chemistry
572.6
QP601.M49 / v.182