MARC details
| 000 -LEADER |
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| fixed length control field |
06531cam a2200625 a 4500 |
| 001 - CONTROL NUMBER |
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22543482 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20201215152917.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
900823s1990 cau b 000 0 eng |
| 010 ## - LIBRARY OF CONGRESS CONTROL NUMBER |
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| Canceled/invalid LC control number |
54009110 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
0121820831 (hardcover : alk. paper) : 0122135857 (comb bound : alk. paper) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
0122135857 (comb bound : alk. paper) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
9780121820831: |
| Terms of availability |
No price |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
NLM |
| Transcribing agency |
NLM |
| Modifying agency |
AGL |
| -- |
IPL |
| -- |
ZHM |
| -- |
VET |
| -- |
MUQ |
| 070 0# - NATIONAL AGRICULTURAL LIBRARY CALL NUMBER |
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| Classification number |
QP601.M49 |
| Item number |
v.182 |
| 072 #0 - SUBJECT CATEGORY CODE |
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| Subject category code |
X300 |
| 072 #7 - SUBJECT CATEGORY CODE |
|---|
| Subject category code |
PSB |
| Source |
thema |
| 072 #7 - SUBJECT CATEGORY CODE |
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| Subject category code |
PDN |
| Source |
thema |
| 072 #7 - SUBJECT CATEGORY CODE |
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| Subject category code |
PN |
| Source |
thema |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
572.6 |
| 245 00 - TITLE STATEMENT |
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| Title |
Guide to protein purification / |
| Statement of responsibility, etc. |
edited by Murray P. Deutscher. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
San Diego : |
| Name of publisher, distributor, etc. |
Academic Press, |
| Date of publication, distribution, etc. |
c1990. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xxix, 894 p. : |
| Other physical details |
ill. ; |
| Dimensions |
25cm. ; |
| Accompanying material |
pbk. comb binding |
| 490 #0 - SERIES STATEMENT |
|---|
| Series statement |
Methods in enzymology ; |
| Volume/sequential designation |
v. 182 |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Includes indexes. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Includes bibliographical references. |
| 505 #0 - FORMATTED CONTENTS NOTE |
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| Title |
Why purify enzymes? / Arthur Kornberg. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section I Developing Purification Procedures] |
| Title |
Strategies and considerations for protein purification / Stuart Linn. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section II General Methods for Handling Proteins and Enzymes] |
| Title |
Setting up a laboratory / Murray P. Deutscher -- |
| -- |
Buffers: principles and practice / Vincent S. Stoll & John S. Blanchard -- |
| -- |
Measurement of enzyme activity / Edward F. Rossomando -- |
| -- |
Quantitation of protein / Christa M. Stoscheck -- |
| -- |
Concentration of proteins and removal of solutes / Thomas Pohl -- |
| -- |
Maintaining protein stability / Murray P. Deutscher. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section III Optimization of Starting Materials] |
| Title |
Overproduction of proteins in Escherichia coli: vectors, hosts and strategies / Asis Das -- |
| -- |
Overexpression of proteins in eukaryotes / Margaret K. Bradley -- |
| -- |
Secretion of heterologous proteins in Escherichia coli / I. Barry Holland et al. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section IV Preparation of Extracts and Subcellular Fractionation] |
| Title |
Preparation of extracts from prokaryotes / Millard Cull and Charles S. McHenry -- |
| -- |
Preparation of extracts from yeast / S. Michal Jazwinski -- |
| -- |
Preparation of extracts from plants / Peter Gegenheimer -- |
| -- |
Preparation of extracts from higher eukaryotes / John David Dignam -- |
| -- |
Isolation of subcellular organelles / Brian Storrie & Edward A. Madden -- |
| -- |
Preparation of membrane fractions / Juris Ozols. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section V Solubilization Procedures] |
| Title |
Detergents: an overview / Judith M. Neugebauer -- |
| -- |
Solubilization of native membrane proteins / Leonard M. Hjelmeland -- |
| -- |
Solubilization of protein aggretates / Fiona A. O. Marston & Donal L. Hartley -- |
| -- |
Removal of detergents from membrane proteins / Leonard M. Hjelmeland. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section VI Purification Procedures: Bulk Methods] |
| Title |
Precipitation techniques / Sasha Englard & Sam Seifter -- |
| -- |
Precipitation of proteins with polyethylene glycol / Kenneth C. Ingham. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section VII Purification Procedures: Chromatographic Methods] |
| Title |
Ion-exchange chromatography / Edward F. Rossomando -- |
| -- |
Gel filtration / Earle Stellwagen -- |
| -- |
Protein chromatography on hydroxyapatite columns / Marina J. Gorbunoff -- |
| -- |
Hydrophobic chromatography / Robert M. Kennedy -- |
| -- |
Chromatography on immobilized reactive dyes / Earle Stellwagen -- |
| -- |
Affinity chromatography: general methods / Steven Ostrove -- |
| -- |
Affinity chromatography: specialized techniques / Steven Ostrove & Shelly Weiss -- |
| -- |
Chromatofocusing / Lallan Giri -- |
| -- |
High-performance liquid chromatography: effective protein purification by various chromatograpic modes / Roman M. Chicz & Fred E. Regnier. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section VIII Purification Procedures: Electrophoretic Methods] |
| Title |
One-dimensional gel electrophoresis / David E. Garfin -- |
| -- |
Protein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis / Bonnie S. Dunbar, Hitomi Kimura & Therese M. Timmons -- |
| -- |
Isoelectric focusing / David E. Garfin -- |
| -- |
Gel staining techniques / Carl R. Merril -- |
| -- |
Evolution of protein from gels / Michael G. Harrington. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section IX Specialized Purification Procedures] |
| Title |
Purification of membrane proteins / Thomas C. Thomas & Mark G. McNamee -- |
| -- |
Purification of DNA-binding proteins by site-specific DNA affinity chromatography / Shlomo Eisenberg et al -- |
| -- |
Purification of glycoproteins / Craig Gerard -- |
| -- |
Purification of multienzyme complexes / Paul A. Srere & Christopher K. Mathews. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section X Characterization of Purified Proteins] |
| Title |
Determination of purity / David G. Rhodes & Thomas M. Laue -- |
| -- |
Determination of size, molecular weight and presence of subunits / Thomas M. Laue & David G. Rhodes -- |
| -- |
Amino acid analysis / Juris Ozols -- |
| -- |
Limited N-terminal sequence analysis / Paul Matsudaira -- |
| -- |
Peptide mapping / Ralph C. Judd -- |
| -- |
Analysis for protein modifications and nonprotein cofactors / Sam Seifter & Sasha Englard -- |
| -- |
Protein crystallization / David Ollis & Stephen White. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section XI Immunological Procedures] |
| Title |
Preparation of polyclonal antibodies / Bonnie S. Dunbar & Eric D. Schwoebel -- |
| -- |
Preparation of monoclonal antibodies / Bonnie S. Dunbar & Sheri M. Skinner -- |
| -- |
Protein blotting and immunodetection / Therese M. Timmons & Bonnie S. Dunbar -- |
| -- |
Immunoprecipitation of proteins / Gary L. Firestone & Sandra D. Winguth -- |
| -- |
Immunoassays / Charles W. Parker. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section XII Additional Techniques] |
| Title |
Radiolabeling of proteins / Charles W. Parker -- |
| -- |
Using purified protein to clone its gene / John M. Wozney -- |
| -- |
Computer analysis of protein structure / Patrick Argos. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Section XIII Concluding Remarks] |
| Title |
Rethinking your purification procedure / Murray P. Deutscher. |
| 505 #0 - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
[Appendix Personal Perspectives on Enzyme Purification] |
| Title |
The private life of DNA polymerase I / Arthue Kornberg -- |
| -- |
Purification and crystallization of transaldolase from candida utilis / B. L. Horecker & O. Tsolas -- |
| -- |
Discovery of glutamine synthetase cascade / Earl R. Stadtman -- |
| -- |
Discovery, resolution, purification and function of elongation factors / Kivie Moldave. |
| 590 ## - LOCAL NOTE (RLIN) |
|---|
| a |
81.74 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Proteins |
| General subdivision |
Purification |
| 9 (RLIN) |
47746 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Proteins |
| General subdivision |
Isolation & purification |
| 9 (RLIN) |
68463 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Enzymes. |
| 9 (RLIN) |
68464 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Science. |
| Source of heading or term |
ukslc |
| 9 (RLIN) |
68465 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Biochemistry |
| Source of heading or term |
thema |
| 9 (RLIN) |
68466 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Scientific equipment, experiments & techniques |
| Source of heading or term |
thema |
| 9 (RLIN) |
34492 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Chemistry |
| Source of heading or term |
thema |
| 9 (RLIN) |
68467 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Deutscher, Murray P. |
| 9 (RLIN) |
29809 |
| 902 ## - LOCAL DATA ELEMENT B, LDB (RLIN) |
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| a |
140901 |
| 903 ## - LOCAL DATA ELEMENT C, LDC (RLIN) |
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| a |
MARS |
| 907 ## - LOCAL DATA ELEMENT G, LDG (RLIN) |
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| a |
.b10195695 |
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cgen |
| c |
- |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Suppress in OPAC |
0 |
| 998 ## - LOCAL CONTROL INFORMATION (RLIN) |
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| Operator's initials, OID (RLIN) |
1 |
| Cataloger's initials, CIN (RLIN) |
030922 |
| First Date, FD (RLIN) |
m |
| Local |
a |
| -- |
- |
| -- |
0 |